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Cultivar description

Cultivar detection

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Cultivar detection


For a long period, the identification of the apricot cultivars was based on pomological, morphological and horticultural traits (IBPGR 1984). Molecular markers like isoenzymes (Badenes et al. 1996) showed low levels of polymorphism and variability among cultivars. DNA-based markers provide a new possibility for evaluating biodiversity among plant genome (Karp et al. 1996). Random amplification polymorphic DNAs (RAPDs) (Gogorcena andParfitt 1994; Zhebentyayeva and Sivolap 2000; Hormaza 2001) and Restriction fragment length polymorphism (RFLPs) markers were developed for apricots (De Vicente et al. 1998), however both methods showed to have some limitations.

Among all available markers, nuclear simple sequence repeats (SSRs or microsatellites) are a widely used class of genetic markers for population studies and genetic diversity assessment (Fossati et al. 2003). The abundance of microsatellites in many plant species, codominant Mendelian inheritance, high level of polymorphism, and easy detection by PCR and electrophoretic methods have made them the genetic markers of choice (Powell et al. 1996; Morgante and Olivieri 1993).

Homologous microsatellites were recently made available from genomic DNA and appeared more polymorphic, when tested on European accessions (Lopes et al. 2002; Hagen et al. 2004; Messina 2004).

Cultivar identity and phylogenetic origin in apricot was confirmed using AFLPs (Hagen et al. 2002, Geuna et al. 2003) or peach microsatellites primers (Hormaza 2002; Romero et al. 2003; Zhebentyayeva et al. 2003), until SSRs were isolated from apricots (Lopes et al. 2002; Messina 2004).

In our study, 10 different primer combinations Table), originally developed for apricot SSR loci and representing different regions of the apricot genome, were used for amplification of DNA from different apricot cultivars.




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last edit 28.5.2006