Plant Biotechnology Unit
at the IAM, BOKU
Research projects
Molecular processes of root induction in woody plants.
Molecular processes of root induction in woody plants.
Duration: 1995-1997; 1997-1999
Supported by: Austrian Science Foundation (FWF)
In the micropropagation of woody species recalcitrance to regenerate roots is one of the major problems. The formation of roots depends on many endogenous and exogenous factors. In this project molecular events during induction of root formation in shoot cultures of Malus domestica are investigated. For this purpose a model system allowing a short induction signal, a distinct response compared to the untreated control, as many as possible reacting cells within the explant has been elaborated. The protein patterns determined with 2D-electrophoresis and Confocal Laser Scanning Microscopy allowing analysis on living objects should provide more evidence about the temporary pattern of root induction. This should provide evidence for the optimal time frame for the isolation of mRNA and the subsequent cloning steps. Genes, which are specific for the root induction phase, are isolated and characterized.
Rev.1.0 by Siegfried Huss